|
KMID : 1100720150350040454
|
|
Annals of Laboratory Medicine
2015 Volume.35 No. 4 p.454 ~ p.457
|
|
|
Direct Identification of Staphylococcus aureus and Determination of Methicillin Susceptibility From Positive Blood-Culture Bottles in a Bact/ALERT System Using Binax Now S. aureus and PBP2a Tests
|
|
Heraud Sandrine
Freydiere Anne-Marie
Doleans-Jordheim Anne
Bes Michele
Tristan Anne
Vandenesch Francois
Laurent Frederic
Dauwalder Olivier
|
|
|
Abstract
|
|
|
|
Staphylococcus aureus bacteremia is associated with high mortality and morbidity, requiring prompt and appropriate antimicrobial treatment. Therefore, it is important to detect methicillin-resistant S. aureus (MRSA) rapidly from blood cultures. Two immunochromatographic tests, BinaxNow S. aureus and BinaxNow PBP2a, were directly applied to 79 Bact/Alert bottles that were positive for Gram positive cocci in cluster aggregations. Sensitivity and specificity for the identification of S. aureus and determination of methicillin resistance were 94% and 87%, and 100% and 100%, respectively, with less than 30 min of performance time. These tests are efficient and rapid; these tests are valuable alternatives to more sophisticated and expensive methods used in the diagnosis of MRSA bacteremia.
|
|
|
KEYWORD
|
|
|
Staphylococcus aureus, Methicillin resistance, Blood culture, Accuracy, Immunochromatographic test
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
   
|